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Thermo Scientific™ GeneJET™ Plant RNA Purification Mini Kit

Perform silica membrane-based total RNA purification from leaves, roots, sprouts and other parts of various plants. Provided in a convenient spin-column format.

Manufacturer:  Thermo Scientific™ K0801

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Catalog No. FERK0801


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Requires : Dithiothreitol (DTT, #R0861)

Description

Description

GeneJET Plant RNA Purification Mini Kit is designed for rapid and efficient purification of high quality total RNA from wide variety of plant species and tissue types.

The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis. Purified high quality RNA can be used in a wide range of downstream applications, such as RT-PCR, RT-qPCR, Northern blotting and other RNA-based analysis. RNA yields vary between different species, tissues, and age of tissue sample. See Table 1 for typical total RNA yields from various sources.

The kit utilizes silica based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol chloroform extractions or time consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis. Purified high quality RNA can be used in a wide range of downstream applications such as RT-PCR, RT-qPCR, Northern blotting and other RNA based analysis. RNA yields vary between different species, tissues and age of tissue sample.
  • Fast: Procedure can be completed in 20 minutes following cell lysis

  • Convenient: Protocol does not include clogging prone lysate filtration step

  • High yield: Up to 65µg of high purity and high integrity RNA from 50mg plant sample

  • Compatible with wide variety of samples (leaves, roots, sprouts and other parts of various plants)

Includes:

Plant RNA Lysis Solution, Wash Buffer WB 1 (concentrated), Wash Buffer 2 (concentrated), Water, nuclease-free, GeneJET™ RNA Purification Columns (pre-assembled with collection tubes), Collection tubes (2 mL), Collection tubes (1.5 mL), Detailed Protocol

Recommended for:

Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including: RT-PCR, RT-qPCR, Northern blotting, nuclease protection assay.

Specifications

Specifications

GeneJET™ Plant RNA Purification Mini Kit
Plant RNA Lysis Solution, Wash Buffer WB 1 (concentrated), Wash Buffer 2 (concentrated), Water, nuclease-free, GeneJET™ RNA Purification Columns (pre-assembled with collection tubes), Collection tubes (2 mL), Collection tubes (1.5 mL), Detailed Protocol
Store at room temperature (15-20°C).
Fast extraction of high purity RNA suitable for all conventional molecular biology procedures, including: RT-PCR, RT-qPCR, Northern blotting, nuclease protection assay.
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