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Thermo Scientific™ GeneJET™ Plasmid Maxiprep Kit

Perform large-scale isolation of high-quality plasmid DNA from recombinant E. coli cultures with this kit.

Manufacturer:  Thermo Scientific™ K0491

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Catalog No. FERK0491


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Description

Description

GeneJET Plasmid Maxiprep Kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription, and transfection of robust cell lines.

The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription and transfection of robust cell lines.
  • Efficient: High yields up to 750µg of high quality plasmid DNA from 250mL of bacterial culture

  • High concentration of DNA purified: 400ng/µl

  • Convenient: Spin column format, centrifuge or vacuum protocols

  • Fast: Procedure takes 60min, considerably faster than using gravity flow columns

Includes:

Resuspension Solution, Lysis Solution, Neutralization Solution, Endotoxin Binding Reagent, Wash Solution I (concentrated), Wash Solution II (concentrated), RNase A Solution, Elution Buffer (10mM Tris-HCl, pH 8.5), GeneJET™ Maxi Purification Columns pre-assembled with collection tubes (50mL), Collection tubes (50mL)

Recommended for:

Transfection of robust cell lines, FastDigest™, restriction enzyme digestion, automated fluorescent sequencing, PCR, cloning, transformation,in vitro transcription.

Specifications

Specifications

400ng/µl
Resuspension Solution, Lysis Solution, Neutralization Solution, Endotoxin Binding Reagent, Wash Solution I (concentrated), Wash Solution II (concentrated), RNase A Solution, Elution Buffer (10mM Tris-HCl, pH 8.5), GeneJET™ Maxi Purification Columns pre-assembled with collection tubes (50mL) and Collection tubes (50mL).
Thermo Scientific™ GeneJET Plasmid Maxiprep Kit
Transfection of robust cell lines, FastDigest™, restriction enzyme digestion, automated fluorescent sequencing, PCR, cloning, transformation,in vitro transcription.
10 Preps
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