Thermo Scientific™ GeneJET Whole Blood Genomic DNA Purification Mini Kit

Perform silica membrane-based genomic DNA purification from fresh, frozen anticoagulated mammalian blood, aged blood, buffy coat, bone marrow and body fluids.

Manufacturer:  Thermo Scientific™ K0781

Catalog No. FERK0781

Description

GeneJET Whole Blood Genomic DNA Purification Mini Kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation. The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting, and enzymatic reactions.

The kit utilizes silica based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions or time consuming alcohol precipitation.The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30kb in size.

• Efficient: High yields of high molecular weight genomic DNA

• Fast: 20 minute procedure following cell lysis

• Convenient: Spin columns are capped and assembled with collection tubes

• Pure: DNA free of enzyme inhibitors, RNA and proteins, ready to use for all demanding applications, validated in realtime PCR, endpoint PCR, multiplex PCR and restriction enzyme digests

• Compatible: Suitable for wide variety of samples, like fresh or frozen anticoagulated mammalian blood (treated with EDTA, Citrate or Heparine), aged blood, buffy coat, bone marrow, body fluids

Includes:

Proteinase K Solution, Lysis Solution, Wash Buffer WB I (concentrated), Wash Buffer II (concentrated), Elution Buffer, GeneJET™ Purification Columns (pre-assembled with collection tubes), Collection tubes, Detailed Protocol

Recommended for:

Isolated genomic DNA is ideal for use in common molecular biology procedures, including PCR and qPCR, FastDigest™ and conventional restriction digestion and Southern blotting.

Specifications

• Column Type: Spin Column
• Isolation Technology: Spin Column
• Scale: Mini
• High-throughput Compatibility: Not High-throughput Compatible (Manual)
• Product Line: GeneJET
• Quantity: 50 Reactions
• Test Time: <20 min

• Format: Spin Column
• Sample Type: Blood
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Cloning, Southern Blotting, Sequencing, PCR
• No. of Reactions: 50
• Product Type: Genomic DNA Purification Kit
• Target: Genomic DNA