Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ GsuI (BpmI) Available on GSA/VA Contract for Federal Government customers only.

The GsuI (BpmI) restriction enzyme recognizes CTGGAG(16/14)^ sites and cuts best at 30°C in B buffer (Isoschizomers: BpmI).

Manufacturer:  Thermo Scientific™ ER0461

 View more versions of this product

Catalog No. FERER0461


Add to cart

Description

Description

Lambda DNA digested with GsuI (BpmI), 1% agarose, 25 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'...C T G G A G (N)16▵...3'

3'...G A C C T C (N)14▵...5'

Conditions for 100% Activity:

  • 1X Buffer B: 10mM Tris-HCl (pH 7.5 at 37°C), 10mM MgCl2 and 0.1mg/mL BSA
  • Incubate at 30°C

Storage Buffer:

  • GsuI is supplied in:10mM potassium phosphate (pH 7.5 at 25°C), 1mM EDTA, 1mM DTT, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

  • Dam: never overlaps —no effect
  • Dcm: may overlap —blocked
  • CpG: never overlaps —no effect
  • EcoKI: never overlaps —no effect
  • EcoBI: never overlaps —no effect

Note:

  • Incubation at 37°C results in 70% activity
  • GsuI requires only Mg2 + for its activity, but is stimulated by S-adenosylmethionine
  • 0.01mM S-adenosylmethionine gives a 2-fold increase in activity
  • GsuI may remain associated with the cleaved DNA
  • This may cause DNA band shifting during electrophoresis
  • To avoid atypical DNA band patterns, use the 6X DNA Loading Dye and SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis
  • GsuI is blocked by overlappingdcm methylation
  • To avoiddcm methylation, use adam,dcm- strain such as GM2163 (#M0099)

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

Specifications

GsuI (BpmI)
5U/μL
100U
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit