Thermo Scientific™ Lambda DNA/EcoRI+HindIII Marker 3 

Perform sizing and approximate quantification of large double-stranded DNA fragments in agarose gels with this ladder that consists of 13 sharp bands.

Manufacturer:  Thermo Scientific™ SM0192

Catalog No. FERSM0192

Description

Lambda DNA/EcoRI+HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientitfic restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

Lambda DNA/EcoRI+HindIII Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, and it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction.

Lambda DNA/EcoRI+HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Fermentas PureExtreme restriction enzyme(s), purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

Lambda DNA/EcoRI+HindIII Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reactio

• Sizing and approximate quantification of large DNA fragments
• Sharp bands
• Supplied with loading dye for sample DNA

Note:

• The cohesive ends (the 12 nt cos site of lambda DNA) of fragments indicated™ may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5 min. and then cooling on ice for 3 min.
• The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of respective DNA sequences.

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Specifications

• Kit Contents: 6X DNA Loading Dye, 10 x 1mL
• Quantity: 25 × 50μg