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Thermo Scientific™ Maxima SYBR Green/ROX qPCR Master Mix (2X) Available on GSA/VA Contract for Federal Government customers only.

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Manufacturer:  Thermo Scientific™ K0221

 View more versions of this product

Catalog No. FERK0221


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Description

Description

The master mixes include Maxima Hot Start Taq DNA Polymerase and dNTPs in an optimized PCR buffer. Only template and primers need to be added. The SYBR Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.
Maxima Hot Start Taq DNA Polymerase in combination with an optimized buffer ensures PCR specificity and sensitivity. dUTP is included in the mix for optional carry-over contamination control using uracil DNA glycosylase (UDG). The use of Maxima SYBR Green qPCR Master Mixes in real-time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral, and cDNA templates. Maxima SYBR Green qPCR Master Mixes are compatible with most real-time thermal cyclers.

  • Specificity - Maxima Hot Start Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers
  • Sensitivity - detects low copy number targets
  • Wide linear range - accurate quantification across 9 orders of magnitude
  • Reproducibility and convenience - ready-to-use 2X master mix

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Specifications

Specifications

Real time PCR (qPCR), gene expression, siRNA validation, genotyping and pathogen detection
200 Reactions
Store at -20°C.
7000 System, 7300 System, 7700 System, 7900HT System, StepOne™ System, StepOnePlus™ System
Standard
2 x 1.25mL Maxima SYBR Green/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with ROX passive reference dye, sufficient for 200 x 25μL reactions; 2 x 1.25mL Nuclease-Free Water
2 x 1.25mL
Detection Method: SYBR; PCR Method: qPCR
DNA, dsDNA
Documents
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For Research Use Only. Not for use in diagnostic procedures.