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Thermo Scientific™ Rapid DNA Ligation Kit Available on GSA/VA Contract for Federal Government customers only.

Enable fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

Manufacturer:  Thermo Scientific™ K1423

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Catalog No. FERK1423


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Description

Description

Rapid DNA Ligation Kit enables fast sticky-end or blunt-end DNA ligation in only 5 minutes at room temperature.

The kit contains T4 DNA ligase and a specially-formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation with the TransformAid Bacterial Transformation Kit or with other conventional transformation procedures.

The kit contains T4 DNA ligase and a specially formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is equal to that obtained with T4 DNA ligase in a standard 1 hour ligation. The ligation reaction mixture can be used directly for bacterial transformation with the TransformAid Bacterial Transformation Kit or with other conventional transformation procedures.
  • Fast—ligation of either sticky- or blunt-end DNA in only 5 minutes
  • Convenient—reaction mixture can be used directly for bacterial transformation

Recommended for:

Routine cloning experiments; Blunt-end cloning; Library construction; TA cloning.

Note:

Lyophilized bacterial strains GM2163 (†M0099) and JM107 (†M0109) can be provided free of charge upon request, with any purchase. Prior to electroporation it is necessary to inactivate T4 DNA ligase (1) by chloroform extraction.

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Specifications

Specifications

Routine cloning experiments; Blunt-end cloning; Library construction; TA cloning.
Rapid DNA Ligation Kit
150 reactions
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