Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit

Perform efficient synthesis of first strand cDNA from RNA templates with this complete synthesis system.

Supplier:  Thermo Scientific™ K1632

Catalog No. FERK1632

Description

RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit includes RevertAid H Minus Reverse Transcriptase, which has a point mutation that completely eliminates RNase H activity. Therefore, it does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA.

Highlights

• High yields of full-length first strand cDNA up to 13 kb
• Increased reaction temperatures in the range of 42 to 55°C
• Supplied with the recombinant RiboLock RNase Inhibitor
• Complete kit - oligo(dT)₁₈ and random hexamer primers included with the kit

Specifications

• Content And Storage:

  • RevertAid H Minus Reverse Transcriptase
  • RiboLock RNase Inhibitor
  • 5X Reaction Buffer
  • dNTP Mix
  • Oligo(dT)₁₈ Primer
  • Random Hexamer Primer
  • Control RNA
  • Control Primers
  • Nuclease-free water
  
  Store at –20°C.
  
  

• Format: First-Strand cDNA Synthesis Kit
• PCR Method: RT-PCR
• Reaction Speed: 60 min.
• Size (Final Product): Up to 13 kb
• Technique: Reverse Transcription
• Reverse Transcriptase: RevertAid H Minus
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)

• Final Product Type: First-Strand cDNA
• No. of Reactions: 100 Reactions
• Reaction Format: Separate components
• Reagent Type: Reverse Transcription
• Starting Material: RNA
• Optimal Reaction Temperature: 42°C to 45°C
• Ribonuclease H Activity: Reduced