Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Perform efficient synthesis of first strand cDNA from RNA templates with this complete synthesis system.

Supplier:  Thermo Scientific™ K1622

Catalog No. FERK1622

Description

RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation.

The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT)₁₈ and random hexamer primers. The oligo(dT)₁₈ primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of the poly(A) tail, therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.

The kit uses RevertAid Reverse Transcriptase which has lower RNase H activity, compared to AMV reverse transcriptase. The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55°C. The oligo(dT)₁₈ anneals selectively on the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis using RNA without poly(A) tail, e.g., micro RNAs. Gene-specific primers may also be used with the kits. The first strand of cDNA can be directly used as a template in PCR (see RT-PCR protocol), real-time PCR or in second strand cDNA synthesis (see protocol).

• Full-length first strand cDNA up to 13kb
• Increased reaction temperatures in the range of 42° to 50°C
• Supplied with the recombinant RiboLock RNase Inhibitor
• Complete—oligo(dT)₁₈ and random hexamer primers included with the kit

Recommended for:

First strand cDNA synthesis for RT-PCR and real-time RT-PCR (1, 2); Construction of full length cDNA libraries; Generation of probes for hybridization; aRNA synthesis.

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Specifications

• Content And Storage:

  • RevertAid Reverse Transcriptase
  • RiboLock RNase Inhibitor
  • 5X Reaction Buffer
  • dNTP Mix
  • Oligo(dT)₁₈ Primer
  • Random Hexamer Primer
  • Control GAPDH RNA
  • Forward GAPDH Primer (10 μM)
  • Reverse GAPDH Primer (10 μM)
  • Nuclease-free water
  
  Store at –20°C.
  
  

• Format: First-Strand cDNA Synthesis Kit
• PCR Method: RT-PCR
• Reaction Speed: 60 min.
• Size (Final Product): Up to 13 kb
• Technique: Reverse Transcription
• Reverse Transcriptase: RevertAid
• For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)

• Final Product Type: First-Strand cDNA
• No. of Reactions: 100 Reactions
• Reaction Format: Separate components
• Reagent Type: Reverse Transcription
• Starting Material: RNA
• Optimal Reaction Temperature: 42°C
• Ribonuclease H Activity: Yes