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Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit
GSA_VA

Perform efficient synthesis of first strand cDNA from RNA templates with this complete synthesis system.

Supplier:  Thermo Scientific™ K1622

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Catalog No. FERK1622


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Description

Description

RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation.

The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of the poly(A) tail, therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.

The kit uses RevertAid Reverse Transcriptase which has lower RNase H activity, compared to AMV reverse transcriptase. The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA template from degradation. It is fully compatible with reverse transcription reaction, as it maintains activity at temperatures up to 55°C. The oligo(dT)18 anneals selectively on the poly(A) tail of mRNA. Random hexamer primers do not require the presence of poly(A). Therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis using RNA without poly(A) tail, e.g., micro RNAs. Gene-specific primers may also be used with the kits. The first strand of cDNA can be directly used as a template in PCR (see RT-PCR protocol), real-time PCR or in second strand cDNA synthesis (see protocol).
  • Full-length first strand cDNA up to 13kb
  • Increased reaction temperatures in the range of 42° to 50°C
  • Supplied with the recombinant RiboLock RNase Inhibitor
  • Complete—oligo(dT)18 and random hexamer primers included with the kit

Recommended for:

First strand cDNA synthesis for RT-PCR and real-time RT-PCR (1, 2); Construction of full length cDNA libraries; Generation of probes for hybridization; aRNA synthesis.

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Specifications

Specifications

• RevertAid Reverse Transcriptase
• RiboLock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Oligo(dT)18 Primer
• Random Hexamer Primer
• Control GAPDH RNA
• Forward GAPDH Primer (10 μM)
• Reverse GAPDH Primer (10 μM)
• Nuclease-free water

Store at –20°C.

First-Strand cDNA Synthesis Kit
RT-PCR
60 min.
Up to 13 kb
Reverse Transcription
RevertAid
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)
First-Strand cDNA
100 Reactions
Separate components
Reverse Transcription
RNA
42°C
Yes
SDS
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