Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/µL)

A recombinant M-MuLV RT lacking RNase H activity for high yield cDNA synthesis.

Supplier:  Thermo Scientific™ EP0452

Catalog No. FEREP0452

Description

RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 50°C).

Highlights

• High yields of full-length first strand cDNA up to 13 kb
• Optimum activity at 42 to 45°C
• Active up to 55°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)

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Specifications

• Content And Storage:

  • RevertAid H Minus Reverse Transcriptase (200 U/μL)
  • 5X Reaction Buffer
  
  Store at –20°C.
  
  

• Final Product Type: First-Strand cDNA
• Reaction Format: Separate components
• Reagent Type: Reverse Transcription
• Starting Material: RNA
• Quantity: 5 x 10,000 Units
• Ribonuclease H Activity: Reduced

• Concentration: 200 U/μL
• Format: Reverse Transcriptase
• Reaction Speed: 60 min.
• Size (Final Product): Up to 13 kb
• Optimal Reaction Temperature: 42°C to 45°C
• Reverse Transcriptase: RevertAid H Minus