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Thermo Scientific™ T3 RNA Polymerase

Thermo Scientific™ Fermentas T3 RNA Polymerase is a DNA-dependent RNA polymerase with strict specificity for its double-stranded promoter.

Specifications

Description T3 RNA Polymerase
For Use With (Application) Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.
Includes 1mL of 5X Transcription Buffer
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Products 1
Catalog Number Mfr. No. Concentration Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Concentration Quantity Price Quantity & Availability  
FEREP0102
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Thermo Scientific™
EP0102
20U/µL 10,000U This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
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Description

Description

Thermo Scientific™ Fermentas Bacteriophage T3 RNA polymerase is DNA-dependent RNA polymerase with strict specificity for their respective double-stranded promoters.

It catalyzes the 5'=>3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from its promoter.
  • Incorporates modified nucleotides (e.g., aminoallyl-, biotin-, fluorescein-, digoxigenin-labeled nucleotides)

Recommended for:

Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.

Note:

Consensus promoter sequences: AATTAACCCTCACTAAA G GGAGA–T3 TAATACGACTCACTATA G GGAGA–T7 ATTTAGGTGACACTATA G AAGNG–SP6 The position in bold (+1) indicates the first nucleotide incorporated into RNA during transcription. Only bases at this position through +3 are critical for transcription, and they must be a G and a purine base, respectively (9).

Specifications

Specifications

T3 RNA Polymerase
Synthesis of unlabeled and labeled RNA that can be used: for hybridization (1), in vitro RNA translation (2); as aRNA (3), siRNA (4), substrate in RNase protection assays (5), template for genomic DNA sequencing.
1mL of 5X Transcription Buffer
SDS
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