Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ Uracil-DNA Glycosylase (UDG, UNG) Available on GSA/VA Contract for Federal Government customers only.

Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar in DNA and prevents carry-over of DNA in PCR reactions.

Manufacturer:  Thermo Scientific™ EN0362

 View more versions of this product

Catalog No. FEREN0362


Add to cart

Description

Description

Uracil-DNA Glycosylase (UDG, UNG) catalyzes the hydrolysis of the N-glycosylic bond between uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. The enzyme shows no activity on RNA.

  • Active in Fermentas buffers for restriction enzymes and thermophilic polymerases
  • Source: E. coli K12 cells
  • Molecular Weight: 25.6 kDa monomer
Quality Control:
  • The absence of endo-, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests

Source:

  • E.coli K12 cells

Molecular Weight:

  • 25.6kDa monomer

Definition of Activity Unit:

  • One unit of the enzyme catalyzes the release 1 nanomole of uracil from uracil-containing DNA template in 60 min. at 37°C
Storage Buffer: The enzyme is supplied in:
  • 30mM Tris-HCl (pH 7.5), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.05% (v/v) Tween 20 and 50% (v/v) glycerol

10X Reaction Buffer:

  • 200mM Tris-HCl (pH 8.2 at 25°C), 10mM EDTA, 100mM NaCl
Inhibition and Inactivation:
  • Inhibitors: Ugi protein from the Bacillus subtilis phage PBS2, protein p56 from the Bacillus subtilis phage phi29 (7).
  • Inactivated by heating at 95°C for 10 min. Enzyme activity is partially restored at temperatures lower than 55°C. Therefore put PCR products on ice after PCR and load directly on a gel

Recommended for:

Control of carry-over contamination in PCR (2); Glycosylase mediated single nucleotide polymorphism detection (GMPD) (3); Site-directed mutagenesis (4); As a probe for protein-DNA interaction studies (5); SNP genotyping; Cloning of PCR products (6); Generation of single strand overhangs of PCR products and cDNA

Note:

The abasic sites formed in DNA by Uracil-DNA Glycosylase may be subsequently cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites.UDG (UNG) is active in the presence or absence of divalent cations.

This product(s) resides on a Fisher Scientific GSA or VA contract. If you are viewing this page as a nonregistered user, the price(s) displayed is List Price. To view your GSA or VA contract pricing, log in using your account number, or become a registered user by contacting one of our Customer Service teams. You can also view your contract price by searching for this item(s) on GSA Advantage. To place an order, contact Fisher Scientific Customer Service.

Specifications

Specifications

1U/μL
E. coli K12 cells
5 x 200U
Ugi protein from the Bacillus subtilis phage PBS2, protein p56 from the Bacillus subtilis phage phi29 (7).
25.6 kDa monomer
Documents
Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

Use of this enzyme in certain applications may be covered by patents and may require a license.