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Thermo Scientific™ XceI (NspI) (10 U/μL)
Cut at RCATG^Y sites with XceI (NspI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BstNSI, NspI).
Supplier: Thermo Scientific™ ER1472
Description
Lambda DNA digested with XceI (NspI), 0.7% agarose, 32 cleavage sites
conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
5' | R | C | A | T | G ↓ | Y | 3' | ||||
3' | Y ↑ | G | T | A | C | R | 5' |
Thermo Scientific XceI (NspI) restriction enzyme recognizes RCATG^Y sites and cuts best at 37°C in Tango buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion. Isoschizomers: BstNSI, NspI.
In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
Note: For methylation sensitivity, refer to product specifications.
Specifications
10 U/μL | |
XceI (NspI) | |
Yes | |
No | |
Traditional Cloning |
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive | |
10X Buffer Tango | |
37°C | |
Restriction Enzyme |
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For Research Use Only. Not for use in diagnostic procedures.