Edvotek™ Fermentation and Bioprocessing of Chromogenic Proteins

Designed to introduce the basic concepts of fermentation and bioprocessing through the production of GFP protein in a small-scale fermentor. Edvotek™ Fermentation and Bioprocessing of GFP Experiment Kit is for 10 lab groups.

Supplier:  Edvotek™ 305

Catalog No. S29016

Includes: instructions, BactoBeads™ with purple and pink plasmid, LB Growth Media, Ampicillin, IPTG, protein extraction buffer, wash buffer, elution buffer, dry ion exchange matrix, standard protein markers, 50% glycerol solution, protein denaturing solution, Microcentrifuge Tubes, pH paper, centrifuge tubes, chromatography columns, protein Protein InstaStain™, practice gel loading solution, tris-glycine-SDS electrophoresis buffer, transfer pipets, loops

Description

Bioprocessing is the production and isolation of desired products from living cells. In this introduction to bioprocessing, students will use small-scale fermenters to produce chromogenic proteins using Escherichia coli. Protein extracts will then be separated using column chromatography to analyze the success of the fermentation process. Finally, the protein solutions will be examined by SDS polyacrylamide gel electrophoresis to determine the purity of the chromogenic proteins.

Specifications

• Product Type: Fermentation and Bioprocessing of GFP Experiment Kit
• Time Range: Complete in 5-6 hours
• Class Size: 10 Lab Groups

• Includes: Instructions, BactoBeads™ with GFP plasmid, LB Growth Media, Growth Additive, Ampicillin, IPTG, GFP Extraction Buffer, Microcentrifuge Tubes, Syringe, 15 ml Centrifuge tubes, transfer pipettes, loops.
• Content And Storage: Refrigerator and Freezer Storage (some components)