Fibroblasts/Epithelial cells Mouse anti-Human, Clone: D7-FIB, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 2 publications

Catalog Number	Mfr. No.	Quantity
NB600777	Novus Biologicals NB600777	0.1 mg
NB018256	Novus Biologicals NB6007770.025MG	0.025 mg

Description

Fibroblasts/Epithelial cells Monoclonal specifically detects Fibroblasts/Epithelial cells in Human samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin (Negative).

Specifications

• Antigen: Fibroblasts/Epithelial cells
• Concentration: 1.0 mg/mL
• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Frozen)
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Fibroblast Cell Markers
• Immunogen: Human foreskin fibroblasts
• Primary or Secondary: Primary
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

• Clone: D7-FIB
• Dilution: Flow Cytometry 1:50-1:200, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Frozen 1:100, Immunohistochemistry-Paraffin (Negative)
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG2a
• Purification Method: Protein G purified
• Test Specificity: NB600-777 recognizes a 112kD molecule expressed on the cell surface of human fibroblasts and epithelial cells. In peripheral blood the antibody stains myeloid cells and a very small number of lymphocytes. Studies upon the antigen have shown it to be sensitive to SDS, but resistant to trypsin, tunicamycin and collagenase. In immunohistological studies the antibody has also been found to bind to epithelium, myoepthelium, smooth muscle and some leucocytes. D7-FIB has been shown to be useful as a cell membrane marker to characterize chondrocyte differentiation giving a positive reaction with dedifferentiated human chondrocytes, and negative with differentiated chondrocytes (3). This product is routinely tested in flow cytometry on the KG1 cell line.

For Research Use Only