Firefly luciferase Monoclonal Antibody (LUC-Y), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA180225

Catalog No. PIMA180225

Description

Membrane permeabilization is required for flow cytometry applications. In Western Blot , MA1-80225 detects a band at ∽55-60 kDa using 1 µg of purified luciferase. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL. Mouse anti Luciferase antibody, clone LUC-Y detects the expression of the luciferase gene of the North American firefly Photinus pyralis in cells and tissues.

Luciferase is a luminescent enzyme produced by Photinus pyralis, also referred to as the common eastern firefly or North American firefly. It is known for its role as a reporter protein and can be used to quantify expression levels from promoters in tissue culture and in vivo. Luciferase catalyses the oxygenation of luciferin using ATP and oxygen to produce oxyluciferin, a highly unstable, singlet-excited compound that emits light upon relaxation to its ground state. Bioluminescence assay systems have become increasingly used in biology and medical research laboratories in addition to (or as alternatives to) fluorescence and chemiluminescence detection strategies. Luciferase enzymes isolated from different animal species have inherent variability in light emission, allowing two or more luciferase enzymes to be used in combination for multiplex analyses, including in vivo imaging, cell viability and single and dual-spectral luciferase reporter assays.

Specifications

• Antigen: Firefly Luciferase
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Gene Alias: Luciferin 4-monooxygenase; Firefly Luciferase; Green Luciferase; Japanese firefly; Genji firefly; Photinus pyralis
• Immunogen: Recombit luciferase expressed in E. Coli
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: 4°C or -20°C if preferred
• Form: Liquid

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Frozen), Western Blot
• Clone: LUC-Y
• Conjugate: Unconjugated
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Target Species: Luciferase
• Product Type: Antibody
• Isotype: IgG1