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Fisher BioReagents™ AMV Reverse Transcriptase (Purif. Avian Myeloblastosis Virus Particles)

Specifications

Concentration 5 to 10U/μL
Product Type AMV Reverse Transcriptase
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Catalog Number Quantity Price Quantity & Availability  
BP3207-1
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This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
BP32075
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This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
Description

Description

  • Description: AMV (Avian Myeloblastosis Virus) Reverse Transcriptase is a DNA polymerase that uses DNA, RNA or RNA:DNA hybrids as templates. It requires a primer as well as Mg2+ or Mn2+ for activity. The avian enzyme possesses an intrinsic ribonuclease H activity. Both nonionic detergents and sulfhydryl compounds stabilize the enzyme activity in vitro.
  • Application: AMV Reverse Transcriptase is used in first and second strand synthesis of cDNA, primer extensions and RNA sequencing and preparation of labeled hybridization probes.
  • Storage Buffer Components: 0.2M potassium phosphate (pH 7.2), 0.2% Triton™ X-100, 2mM DTT and 50% gycerol.
  • Provided with 5X Reaction Buffer: 250mM Tris-HCl (pH 8.3 at 25°C), 250mM KCl, 50mM MgCl2, 2.5mM spermidine, and 50mM DTT. Note: Buffer provided is not compatible with PCR.
  • Unit Definition: One unit is defined as the amount of enzyme which catalyzes the incorporation of 1nmol of dTTP into acid-insoluble form in 10 minutes at 37°C in 50mM Tris-HCl (pH 8.3), 40mM KCl, 8.75mM MgCl2, 10mM DTT, 0.1mg/ml BSA, 1mM radiolabeled dTTP, and 0.25mM poly(A)400 and 0.25mM oligo (dT)50.
  • Storage: -20°C

Specifications

Specifications

5 to 10U/μL
AMV Reverse Transcriptase
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