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Fisher BioReagents™ DNA Polymerase I Large (Klenow) Fragment (Recombinant E. coli Strain)

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Catalog No. BP32015

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  • Description: The DNA Polymerase I Large (Klenow) Fragment lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I but retains the 5' to 3' polymerase, the 3' to 5' exonuclease and strand displacement activities
  • Application: DNA Polymerase I Large (Klenow) Fragment is used for filling-in or labeling 3' ends of double-stranded DNA, second strand cDNA synthesis, and DNA sequencing by the dideoxy method
  • Storage Buffer Components: 0.05M Tris-HCl (pH 7.5), 1mM DTT, 0.1mM EDTA and 50% (v/v) glycerol
  • Provided with 10X Reaction Buffer: 500mM Tris-HCl (pH 7.2), 100mM MgSO4, and 1mM DTT
  • Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of total deoxyribonucleotides into TCA-insoluble form in 30 minutes at 37°C in 67mM potassium phosphate, (pH 7.5), 6.7mM MgCl2, 1mM DTT, 133µM activated calf thymus DNA and 33µM dATP, dCTP, dGTP, and radiolabeled dTTP
  • Storage: -20°C



DNA Polymerase I
5 to 10U/μL
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