Fisher BioReagents™ DNA Polymerase I Large (Klenow) Fragment (Recombinant E. coli Strain)

Catalog No. BP32015

Description

• Description: The DNA Polymerase I Large (Klenow) Fragment lacks the 5' to 3' exonuclease activity of intact DNA Polymerase I but retains the 5' to 3' polymerase, the 3' to 5' exonuclease and strand displacement activities
• Application: DNA Polymerase I Large (Klenow) Fragment is used for filling-in or labeling 3' ends of double-stranded DNA, second strand cDNA synthesis, and DNA sequencing by the dideoxy method
• Storage Buffer Components: 0.05M Tris-HCl (pH 7.5), 1mM DTT, 0.1mM EDTA and 50% (v/v) glycerol
• Provided with 10X Reaction Buffer: 500mM Tris-HCl (pH 7.2), 100mM MgSO₄, and 1mM DTT
• Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of total deoxyribonucleotides into TCA-insoluble form in 30 minutes at 37°C in 67mM potassium phosphate, (pH 7.5), 6.7mM MgCl₂, 1mM DTT, 133µM activated calf thymus DNA and 33µM dATP, dCTP, dGTP, and radiolabeled dTTP
• Storage: -20°C

Specifications

• Product Type: DNA Polymerase I
• Concentration: 5 to 10U/μL