Fisher BioReagents™ Recombinant DNase 10X RXN Buffer, Optizym™, Fisher BioReagents

Ideal for use in RT-PCR and other RNA applications

Catalog Number	Concentration	Quantity	Storage Buffer
BP32261	2U/μL	1000U	20mM HEPES (7.5 pH), 10mM CaCl2, 10mM MgCl2, 1mM DTT, 50% Glycerol
BP32271	10X	Each	100mM Tris HCl (7.5 pH), 25mM MgCl2, 5mM CaCl2
BP32262	2U/μL	2,000U	20mM HEPES (7.5 pH), 10mM CaCl2, 10mM MgCl2, 1mM DTT, 50% Glycerol

Description

• Efficiently hydrolyzes phosphodiester linkages of double- or single-stranded DNA
• Requires magnesium and calcium for maximal activity
• Tested for RNase activity, protease activity, and in specific performance tests
• Storage Buffer Components: 20mM HEPES (pH 7.5), 10mM CaCl₂, 10mM MgCl₂, 1mM DTT, 50% (v/v) glycerol
• Provided with 10X Reaction Buffer: 100mM Tris-HCl (pH 7.5), 25mM MgCl₂, 5mM CaCl₂
• Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1µg DNA in 10 minutes at 37°C
• Unit present in each tube: 1mL per ampule

Specifications

• Product Type: OPTIZYME™ rDNase I
• Format: Viscous Liquid
• For Use With (Application): RT-PCR, other RNA applications
• Storage Requirements: -20°C