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Fisher BioReagents™ Recombinant DNase 10X RXN Buffer, Optizym™, Fisher BioReagents

Ideal for use in RT-PCR and other RNA applications

Specifications

Product Type OPTIZYME™ rDNase I
Format Viscous Liquid
For Use With (Application) RT-PCR, other RNA applications
Storage Requirements -20°C
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Products 3
Catalog Number Concentration Quantity Storage Buffer Price Quantity & Availability  
Catalog Number Concentration Quantity Storage Buffer Price Quantity & Availability  
BP32261
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2U/μL 1000U 20mM HEPES (7.5 pH), 10mM CaCl2, 10mM MgCl2, 1mM DTT, 50% Glycerol This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
BP32271
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10X Each 100mM Tris HCl (7.5 pH), 25mM MgCl2, 5mM CaCl2 This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
BP32262
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2U/μL 2,000U 20mM HEPES (7.5 pH), 10mM CaCl2, 10mM MgCl2, 1mM DTT, 50% Glycerol This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
N/A
Description

Description

  • Efficiently hydrolyzes phosphodiester linkages of double- or single-stranded DNA
  • Requires magnesium and calcium for maximal activity
  • Tested for RNase activity, protease activity, and in specific performance tests
  • Storage Buffer Components: 20mM HEPES (pH 7.5), 10mM CaCl2, 10mM MgCl2, 1mM DTT, 50% (v/v) glycerol
  • Provided with 10X Reaction Buffer: 100mM Tris-HCl (pH 7.5), 25mM MgCl2, 5mM CaCl2
  • Unit Definition: One unit is defined as the amount of enzyme required to completely degrade 1µg DNA in 10 minutes at 37°C
  • Unit present in each tube: 1mL per ampule
Specifications

Specifications

OPTIZYME™ rDNase I
Viscous Liquid
RT-PCR, other RNA applications
-20°C
SDS
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