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Fisher BioReagents™ Ribonuclease H (Source: Recombinant E. coli Strain)

Catalog No. BP3215-1

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

  • Application: Ribonuclease H is used in removal of the RNA strand prior to second strand cDNA synthesis and analysis of in vitro polyadenylation reaction products.
  • Description: Ribonuclease H (RNase H) from E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA to produce 3'-OH and 5'-P terminated products. It will not degrade single-stranded nucleic acids, duplex DNA or double-stranded RNA.
  • Storage Buffer Components: 20mm HEPES-KOH (pH 7.8), 1mM DTT, 50mM KCl and 50% (v/v) glycerol, and 0.2mg/ml BSA.
  • Unit Definition: One unit is defined as the amount of enzyme required to produce 1nmol acid-soluble ribonucleotides from radiolabeled poly(rA):poly(dT) in 20 minutes at 37°C in 20mM HEPES-KOH (pH 7.8), 50mM KCl, 10mM MgCl2, 1mM DTT, 20μM radiolabeled poly(rA):poly(dT).
  • Storage: -20°C

Specifications

Specifications

Ribonuclease H
0.5 to 2U/μL
SDS
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