Fisher BioReagents™ Ribonuclease H (Source: Recombinant E. coli Strain)
- Application: Ribonuclease H is used in removal of the RNA strand prior to second strand cDNA synthesis and analysis of in vitro polyadenylation reaction products.
- Description: Ribonuclease H (RNase H) from E. coli is an endoribonuclease that specifically hydrolyzes the phosphodiester bonds of RNA hybridized to DNA to produce 3'-OH and 5'-P terminated products. It will not degrade single-stranded nucleic acids, duplex DNA or double-stranded RNA.
- Storage Buffer Components: 20mm HEPES-KOH (pH 7.8), 1mM DTT, 50mM KCl and 50% (v/v) glycerol, and 0.2mg/ml BSA.
- Unit Definition: One unit is defined as the amount of enzyme required to produce 1nmol acid-soluble ribonucleotides from radiolabeled poly(rA):poly(dT) in 20 minutes at 37°C in 20mM HEPES-KOH (pH 7.8), 50mM KCl, 10mM MgCl2, 1mM DTT, 20μM radiolabeled poly(rA):poly(dT).
- Storage: -20°C
|0.5 to 2U/μL|
We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.
Your feedback has been submitted. Fisher Scientific is always working to improve our content for you. We appreciate your feedback.Ok