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Fisher BioReagents™ T3 RNA Polymerase (Source: Recombinant E. coli Strain)

Specifications

Product Type T3 RNA Polymerase
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Products 2
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Catalog Number Quantity Price Quantity & Availability  
BP32065
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This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
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BP3206-1
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This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
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Description

Description

  • Application: T3 RNA Polymerase is used in synthesis of RNA transcripts for hybridization probes, in vitro translation, RNase protection assays and RNA processing substrates.
  • Description: T3 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits extremely high specificity for T3 promoter sequences. The enzyme will incorporate 32P, 35S and 3H-labeled nucleotide triphosphates.
  • Storage Buffer Components: 20mM potassium phosphate (pH 7.7), 100mM NaCl, 1.0mM EDTA, 10mM DTT, 0.1% (v/v) Triton™ X-100 and 50% (v/v) glycerol.
  • Provided with 100mM DTT and Transcription Optimized 5X Buffer: 200mM Tris-HCl (pH 7.9), 30mM MgCl2, 10mM spermidine, and 50mM NaCl.
  • Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 5nmol of CTP into acid-insoluble product in 60 minutes at 37°C in a total volume of 100µL. The reaction conditions are: 40mM Tris-HCl (pH 7.9), 6mM MgCl2, 10mM DTT, 10mM NaCl, 2mM spermidine, 0.05% Tween™-20, 0.5mM each of ATP, GTP, CTP, and UTP, 0.5µCi [3H] CTP, and 2µg supercoiled pSP6/T3 Vector DNA.
  • Storage: -20°C

Specifications

Specifications

T3 RNA Polymerase
SDS
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