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Fisher BioReagents™ T4 Polynucleotide Kinase (Source: Recombinant E. coli Strain)

Specifications

Product Type T4 Polynucleotide Kinase
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BP3212-1
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This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
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BP32125
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1000U This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
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Description

Description

  • Application: T4 Polynucleotide Kinase is used in 5' end-labeling of single- or double-stranded DNA and RNA molecules for use as probes, for sequencing or for DNA-protein footprinting.
  • Description: T4 Polynucleotide Kinase catalyzes the transfer of the γ-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 3'-phosphate group.
  • Storage Buffer Components: 20mM Tris-HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1µM ATP and 50% (v/v) glycerol.
  • Provided with 10X Reaction Buffer: 700mM Tris-HCl (pH 7.6), 100mM MgCl2, and 50mM DTT.
  • Unit Definition: One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of phosphate to the 5'-OH end of an oligonucleotide from [γ-32P]ATP in 30 minutes at 37°C. The reaction conditions are: 40mM Tris-HCl (pH 7.5), 10mM MgCl2, 5mM DTT, 0.1mM [γ-32P] ATP and 0.5mM 5'-OH polynucleotide end concentration.
  • Storage: -20°C

Specifications

Specifications

T4 Polynucleotide Kinase
SDS
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