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R&D Systems™ Fluorescein-NAD

R&D Systems™ Fluorescein-NAD provides a convenient, non-isotopic alternative to radiolabeled NAD for use with enzymes requiring NAD as a substrate or cofactor.

Manufacturer:  R&D Systems™ 467350001

Catalog No. 46-735-0001

This item has been discontinued and is no longer available. Please call customer service for assistance: 1-800-766-7000.

This item is not returnable. View return policy

Description

Description

  • Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • Do not use past expiration date.
  • Provided in solution at a concentration of 250µM in deionized water. There is a 1:1 stoichiometry for incorporation; one Fluorescein label for each NAD molecule.
  • Activity measurements of NAD-requiring enzymes.
  • Assays to identify inhibitors of activators of NAD-requiring enzymes.
  • A working concentration of 5-25µM has been effective in cell staining to determine PARP activity; the working concentration, however, may require prior optimization based on application, cell type, or cell number used.
  • Fluorescein NAD is a highly charged molecule and will not enter live cells without the aid of a cell penetrating peptide.
Specifications

Specifications

250μL
Store the unopened product at -70°C
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