Molecular Probes Fluoresceinyl Glycine Amide (5-(Aminoacetamido)Fluorescein)
Fluoresceinyl Glycine Amide (5-(Aminoacetamido)Fluorescein)
Manufacturer: Molecular Probes A1363
DescriptionThe primary aliphatic amine of 5-(aminoacetamido)fluorescein (fluoresceinyl glycine amide) can be reversibly coupled to aldehydes and ketones to form a Schiff base - which can be reduced to a stable amine derivative by sodium borohydride (NaBH4) or sodium cyanoborohydride (NaCNH3) to form new probes. Carboxylic acids of proteins and other water-soluble biopolymers can be coupled to this molecule in aqueous solution using water-soluble carbodiimides such as EDAC (E2247). The glycine may be the better amine probe for direct carbodiimide-mediated coupling, since it is likely to remain unprotonated at a lower pH than other aliphatic amines.
|Fluoresceinyl Glycine Amide (5-(Aminoacetamido)Fluorescein), 10mg, Orange, Solid, C22H16N2O6 Molecular Formula, 404.38 Molecular Weight, FITC (Fluorescein) Label, Aldehyde, Carboxylic Acid, Ketone Chemical Reactivity, 515/491nm Excitation/Emission Wavelen|
|Labeling chemistry, protein labeling and crosslinking|
|Fluoresceinyl Glycine Amide (5-(Aminoacetamido)Fluorescein)|
|HPLC, 98% at 254nm|
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For Research Use Only. Not for use in diagnostic procedures.