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FOXP3 Monoclonal Antibody (236A/E7), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

$403.00

Specifications

Clone 236A/E7
Concentration 5 μL/Test
Applications Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
Classification Monoclonal
Conjugate Alexa Fluor 488
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Products 1
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-167-57
Encompass_Preferred
View Documents
Invitrogen
53477742
100 Tests
Each of 1 for $403.00
Add to Cart
 
Description

Description

Description: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25bright population. The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque. Applications Reported: This 236A/E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis and IHC. Applications Tested: This 236A/E7 antibody has been pre-titrated and tested using the Foxp3/Transctiption Factor Staining Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 μL (0.

FOXP3 Monoclonal antibody specifically detects FOXP3 in Human, Non-human Primate, Rhesus Monkey samples. It is validated for Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin).
Specifications

Specifications

236A/E7
Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin)
Alexa Fluor 488
Mouse
Human, Non-human Primate, Rhesus Monkey
50943, 574303
Primary
4° C, store in dark, DO NOT FREEZE!
5 μL/Test
Monoclonal
Liquid
RUO
Q9BZS1
IgG1 κ
Affinity chromatography
Antibody
SDS
Documents

Documents

Product Certifications

For Research Use Only.

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