FOXP3 Monoclonal Antibody (236A/E7), eFluor™ 450, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 48477742

Catalog No. 50-112-9020

Description

Description: The 236A/E7 antibody reacts with human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells. Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3 Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25^bright population. The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). Applications Reported: This 236A/E7 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 236A/E7 antibody has been pre-titrated and tested using the Foxp3/Transctiption Factor Staining Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 μL (0.25 μg) per test.

FOXP3 Monoclonal antibody specifically detects FOXP3 in Human, Non-human Primate, Rhesus Monkey samples. It is validated for Flow Cytometry.

Specifications

• Antigen: FOXP3
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9BZS1
• Gene Symbols: Foxp3
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 50943, 574303
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 236A/E7
• Conjugate: eFluor 450
• Gene: Foxp3
• Gene Alias: AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human, Non-human Primate, Rhesus Monkey
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.