Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

Combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.

Catalog Number	Mfr. No.	Quantity
T1027	Invitrogen™ T1027	1000 Assays
T1028	Invitrogen™ T1028	10,000 Assays
T1029	Invitrogen™ T1029	200 Assays

Description

• Homogeneous assay format allows detection of β-galactosidase in presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible
• The kinetics of glow-assay provides a “window” during which measurements may be performed, facilitating HTS applications where assay automation is used
• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems
• Wide dynamic range of β-galactosidase assay enables accurate measurement of enzyme from femtogram to nanogram range
• Assay sensitivity is 100 to 1,000-fold better than either the isotopic/non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric/fluorescent assays for β-galactosidase, providing greater sensitivity than competing assay technologies
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes
• Assay can be completed in about one hour, providing fast assay turnaround

Ideal for Screening

This homogeneous assay is ideally suited for screening applications where assay automation is required. The Gal-Screen system uses Galacton-Star™ chemiluminescent substrate for convenient measurement in a luminometer. Light emission reaches maximum in 60-90 minutes and remains constant for 45-90 minutes.

 

Specifications

• Assay Type: β-Gal (lacZ) Assay
• Compatible Cells: Mammalian Cells
• Detection Method: Chemiluminescent
• For Use With Equipment: Luminometer (Microplate)
• Form: Liquid
• Format: 96-well plate
• Readout: End Point
• Sample Type (Specific): Cell Cultures
• Substrate: Galacton-Star™
• Substrate Properties: Chemical Substrate
• Substrate Type: Beta-Gal Substrate
• Target Enzyme: Beta-Galactosidase
• Technique: Enhanced Chemiluminescence

Specifications

• Product Type: β-Galactosidase Reporter Gene Assay System
• For Use With (Equipment): Luminometer (Microplate)
• Compatible Cells: Mammalian Cells
• Label or Dye: Unconjugated
• Sample Type: Cell Cultures
• Substrate Type: Beta-Gal Substrate
• Target Enzyme: Beta-Galactosidase
• For Use With (Application): Enzyme Assay

• Detection Method: Chemiluminescence
• Assay: β-Gal (lacZ) Assay
• High-throughput Compatibility: High-throughput Compatible
• Readout: End Point
• Substrate: Galacton-Star™
• Substrate Properties: Chemical Substrate
• Product Line: Gal-Screen™, NovaBright™
• Form: Liquid

For Research Use Only. Not for use in diagnostic procedures.