Cytiva GammaBind Plus Sepharose

Binds to all IgG subclasses. Cytiva GammaBind Plus Sepharose is used for lab-scale affinity purification of IgG from a large variety of species and has better binding to rat and mouse IgG subclasses compared to standard GammaBind Sepharose.

Catalog Number	Mfr. No.	Quantity
45-002-366	Cytiva 17088602	25 mL
45-002-365	Cytiva 17088601	5 mL

Description

GammaBind Plus Sepharose may be used to analyze and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium.

• Recombinant Protein G type 3 coupled to Sepharose CL-6B for enhanced binding to rat and mouse IgG subclasses at laboratory scale
• Binds to all IgG subclasses from human, mouse, and rat; binds total IgG from guinea pig, rabbit, goat, cow, sheep, and horse
• Yields up to 18mg/ml mouse IgG
• Available in 5ml and 25ml sizes

GammaBind Plus Sepharose is GammaBind G, Type 3, covalently immobilized to Sepharose CL-6B by malimide linkage. GammaBind G, Type 3, a recombinant form of streptococcal protein G, binds to the Fc region of IgG from a variety of mammalian species. Since only the Fc region is involved in binding, the Fab region is still available for binding antigen.

Specifications

• Flow Rate: >130 cm/h
• Ligand: Recombinant GammaBind G type 3 lacking albumin-binding region
• Particle Size: 90 μm, d_50v is the median particle size of the cumulative volume distribution.
• Content And Storage: 2°C to 8°C, 20% Ethanol