BioVision Inc GAPDH Activity Assay Kit: Glyceraldehyde-3-phosphate Dehydrogenase (GAPDH) Activity Colorimetric Assay Kit: Simple & convenient assay to measure GAPDH activity in animal tissues and cells . Detection limit: 100 µU/mL. 100 Assays.
Manufacturer: BioVision Inc K680100
GAPDH Activity Assay Kit: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC 220.127.116.11) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. Deregulation of GAPDH activity is associated with abnormal cell proliferation and carcinogenesis. Accurate quantitation of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology. BioVision's GAPDH Activity Assay Kit provides a simple and sensitive method for monitoring GAPDH activity in various samples. In this assay, GAPDH catalyzes conversion of GAP into BPG and an intermediate, which reacts with a developer to form a colored product that absorbs maximally at 450 nm. Our high-throughput adaptable assay can detect GAPDH activity as low as 100 ¿U/mL in a variety of samples.
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