Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

GAPDH Mouse anti-Human, Mouse, Non-human primate, Clone: FF26A, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 14952380

 View more versions of this product

Catalog No. 50-112-2789


Add to cart

Description

Description

Description: This FF26A monoclonal antibody reacts with human glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a key component of glycolysis. This 36 kDa enzyme is constitutively expressed in all tissues albeit at different levels. Measurement of GAPDH levels is often performed as a control for protein loading in experiments assessing differential protein expression. The FF26A antibody is crossreactive to mouse GAPDH. Applications Reported: This FF26A antibody has been reported for use in western blotting. Applications Tested: This FF26A antibody has been tested by immunoblotting of cell lysates. This can be used at less than or equal to 1 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.

GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, GAPDH is ubiquitously expressed and displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Studies provide evidence of GAPDH playing an essential part in gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases. Further, GAPDH is involved in other cellular processes ranging from membrane fusion, and neuronal apoptosis in cancer. GAPDH is reported to bind to a variety of other proteins, including the amyloid precursor protein, mutations in which cause some forms of Alzheimer ft.s disease (AD), and the polyglutamine tracts of Huntingtin, the protein product aberrant forms of which are causative of Huntington ft.s disease. Associations between GAPDH, actin and tubulin have also be reported. Since GAPDH is expressed at high levels in most tissues, it is useful as protein loading control in Western Blot analysis.
Specifications

Specifications

GAPDH
Monoclonal
0.5 mg/mL
PBS with 0.09% sodium azide; pH 7.2
P04406, P16858
Glyceraldehyde-3-Phosphate Dehydrogenase, GAPD, G3PD, G3PDH
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human, Mouse, Non-human primate
Western Blot
FF26A
Unconjugated
GAPDH
Liquid
GAPDH
IgG1, kappa
25 μg
4° C
Primary
14433, 2597
Documents
Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit

For Research Use Only.