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Gata-3 Rat anti-Human, Mouse, Porcine, Rhesus Monkey, Clone: TWAJ, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

$129.00 - $344.00

Specifications

Antigen Gata-3
Clone TWAJ
Target Species Human, Mouse, Porcine, Rhesus Monkey
Conjugate Unconjugated
Isotype IgG2b, kappa
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-139-33 Non-distribution product as customer accommodation.
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Invitrogen
14996680
25 μg Each for $129.00
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50-139-34 Non-distribution product as customer accommodation.
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Invitrogen
14996682
100 μg Each for $344.00
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Description

Description

Description: The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors. Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation. Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney. During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal. The Gata-3 is also essential for T cell commitment and survival. In the thymus, expression is found mainly on the CD4 single positive cells. During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation. Alternative splice variants have been reported especially in the MCF7 cell line. The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein. Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set.(cat 00-5523) Crossreactivity in rhesus monkeys has been published. Applications Reported: This TWAJ antibody has been reported for use in immunoblotting (WB) and immunocytochemistry. Applications Tested: This TWAJ antibody has been tested by western blotting of Th2 polarized human or mouse CD4 T cells or MCF7 cell line. This can be used at less than or equal to 2 µg/mL.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.
Specifications

Specifications

Gata-3
Human, Mouse, Porcine, Rhesus Monkey
IgG2b, kappa
Monoclonal
Liquid
Antibody
4° C
TWAJ
Unconjugated
Immunocytochemistry, Immunofluorescence, Western Blot
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO

For Research Use Only.

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