GE Healthcare 2-D Quant Kit
Use for accurate determination of protein concentration in samples prepared for electrophoresis techniques. GE Healthcare Life Sciences 2-D Quant Kit precipitates proteins while leaving interfering substances behind.
Manufacturer: GE Healthcare 80648356
Designed for the accurate determination of protein concentration in samples prepared for electrophoresis techniques. Works by quantitatively precipitating proteins while leaving interfering substances behind. The assay is based on the specific binding of copper ions to protein.
- Works in the presence of 2% SDS, 1% DTT, 8M urea, 2M thiourea, 4% CHAPS, 2% Pharmalyte, and 2% IPG Buffer
- Quantitatively precipitates proteins while leaving interfering substances behind
- Linear response in the range of 0 to 50µg protein, with sample volumes of 1 to 50µL
- Assay time: one hour
2-D electrophoresis, SDS-PAGE, IEF
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|2D Quant kit|
|2D Electrophoresis, SDS-PAGE, IEF|
|1 to 50μL|
|250mL Precipitant, 250mL Co Precipitant, 50mL Copper Solution, 2 x 250mL Color reagent A, 5mL Color reagent B, 5mL BSA standard (2mg/mL)|
|4° to 8°C|
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