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GE Healthcare Activated Thiol Sepharose™ 4B Medium

For reversible coupling of proteins to Sepharose 4B via a glutathione spacer arm

Manufacturer:  GE Healthcare 17064001

Catalog No. 45-000-122


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Description

Description

  • Gel reacts with heavy metal ions and with alkyl and aryl halides
  • Addition reactions occur with compounds containing C=O, C=C, and N=N bonds
  • Less substituted but more porous than Thiopropyl Sepharose 6B
  • Suitable for covalent chromatography of large molecules such as enzymes and nucleic acids
  • Possible to couple proteins lacking thiol groups to thiol gels using the heterobifunctional reagent SPDP, facilitating use of covalent chromatography

Specifications

Specifications

Used for reversible immobilization of molecules containing Thiol groups under mild conditions, optimized for immobilization of large molecules
4% agarose
90μm
15g
1μM Activated Thiol/mL Medium
4% Agarose
2 to 8
2° to 8°C
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