GE Healthcare Amersham AlkPhos Direct ECF Detection Module
Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes
Manufacturer: GE Healthcare RPN3685
AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star or ECF detection can be used. DNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.
- Hybridization stringency can be controlled by elevating temperatures up to 75°C
- Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
- ECF detection for quantitation experiments
- Rapid protocol saves two to three hours over conventional indirect methods
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Each system include the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500cm2: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, ECF detection reagents.
|Sufficient for 25 or 50 labeling reactions and detection of 2500cm2 membrane: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, ECF detection reagents.|
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