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GE Healthcare Amersham™ AlkPhos Direct™ ECF Detection Module Available on GSA/VA Contract for Federal Government customers only.

Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes

Manufacturer:  GE Healthcare RPN3685

Catalog No. 45-000-938


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Description

Description

AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star™ or ECF™ detection can be used. DNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.

  • Hybridization stringency can be controlled by elevating temperatures up to 75°C
  • Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
  • ECF detection for quantitation experiments
  • Rapid protocol saves two to three hours over conventional indirect methods

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Order Info

Each system include the following reagents, sufficient for 25 or 50 labeling reactions and detection of 2500cm2: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, ECF detection reagents.

Specifications

Specifications

Sufficient for 25 or 50 labeling reactions and detection of 2500cm2 membrane: labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, ECF detection reagents.
Each
2500cm2 membrane
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