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GE Healthcare Amersham™ AlkPhos Direct™ Labeling and Detection System with CDP-Star

Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes

Manufacturer:  GE Healthcare RPN3691

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Catalog No. 45-001-776

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Specifications

Description

AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star™ or ECF™ detection can be used. DNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.

  • Hybridization stringency can be controlled by elevating temperatures up to 75°C
  • Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
  • ECF detection for quantitation experiments
  • Rapid protocol saves two to three hours over conventional indirect methods

Description & Specifications

Specifications

Labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star detection reagents
2° to 8°C
All high-sensitivity applications using DNA, RNA, or oligonucleotide probes
Each
5000cm2 membrane
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