GE Healthcare Amersham™ AlkPhos Direct™ Labeling and Detection System with CDP-Star

Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes

Manufacturer: GE Healthcare RPN3690

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Catalog No. 45-001-775

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This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000. 


    Specifications

    Includes Labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer, blocking agent, CDP-Star detection reagents
    Quantity Each
    Storage Conditions 2° to 8°C
    Sufficient For 2500cm2 membrane
    For Use With (Application) All high-sensitivity applications using DNA, RNA, or oligonucleotide probes

    AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star™ or ECF™ detection can be used. DNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.

    • Hybridization stringency can be controlled by elevating temperatures up to 75°C
    • Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
    • ECF detection for quantitation experiments
    • Rapid protocol saves two to three hours over conventional indirect methods

  • Description & Specifications

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