GE Healthcare Amersham AlkPhos Direct Labeling Modules
Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes. AlkPhos Direct Labeling Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase.
Manufacturer: GE Healthcare RPN3680
DescriptionDNA or RNA probes are labeled during a 30-minute reaction and resulting probes can be used without purification. Hybridization stringency can be controlled by temperature as well as salt concentration.
- Hybridization stringency can be controlled by elevating temperatures up to 75°C
- Detection of 60 fg target DNA (single-copy gene) in a genomic Southern blot
- Rapid protocol saves two to three hours over conventional indirect methods
|AlkPhos Direct Labeling and Detection Systems are based on the rapid, direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. CDP-Star or ECF detection can be used.|
|Labeling reagent, crosslinker, control DNA, reaction buffer, hybridization buffer and blocking agent|
|Fast and convenient systems for all high-sensitivity applications using DNA, RNA or oligonucleotide probes|
|AlkPhos Direct Labeling Module|
|2° to 8°C|
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