Cy3 and Cy5 mono-functional maleimides are particularly suitable for the selective labeling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides, and oligonucleotides. They are also useful in antibody labeling since the dye molecules are introduced away from the antibody binding site, increasing the chance that functionality of the labeled molecule will be unimpaired. The protocol is simple and robust, offering an alternative chemistry when either greater selectivity of labeling is required or amine labeling with Cy3 and Cy5 NHS esters is not desirable or appropriate.
Provides an alternative for when CyDye NHS esters are unsuitable.
Dyes are packaged in premeasured amounts and foil-sealed to ensure consistent labelings
For the labeling of biological compounds with CyTM5 mono-functional dye
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