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GE Healthcare Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System

Use for fast and easy detection of medium-to-high-target amounts. GE Healthcare Life Sciences™ Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction

Manufacturer:  GE Healthcare RPN3000

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Catalog No. 45-000-922


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Description

Description

 

    • Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL Direct, Hybond N+, and Hyperfilm ECL.
    • Eliminates handling, waste, and regulatory issues associated with the use of radioactivity.
    • No need to strip blots before reprobing.
    • For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses.
    • Consistent results combining strong signals with very low backgrounds.

 

 

Specifications

Specifications

Colony or plaque screens, dot blots and PCR product analyses
ECL Direct Labeling and Detection System
20 min.
DNA labeling reagent 0.5mL; Glutaraldehyde solution 0.5mL; Water 1.0mL; l Hind III control DNA (10μg/mL) 0.1mL; Gold hybridization buffer 500mL; Blocking agent 25g; ECL detection reagent 1 125mL; ECL detection reagent 2 125mL
2 - 8°C
Labeling 5μg Nucleic Acid
Safety and Handling

Safety and Handling

ShelfLife : 3 Months

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