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GE Healthcare Amersham™ Megaprime™ DNA Labeling System

For labeling DNA probes to specific activities >1 x 109dpm/µg in 10 minutes

Manufacturer:  GE Healthcare RPN1607

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Catalog No. 45-000-810


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Includes: Primer solution; Labeling buffer; Reaction buffer; Enzyme; Standard DNA solution; Carrier DNA solution

Description

Description

Megaprime™ DNA Labeling Systems are used for rapid, random-prime labeling from as little as 25ng substrate DNA.

  • Features cloned Klenow fragment and random nonamers in the random-prime labeling technique
  • No need to remove unincorporated nucleotides before hybridization
  • Protocols for labeling directly from low melting point agarose

Specifications

Specifications

Membrane hybridization
Megaprime DNA Labeling System, dCTP (for use with labeled dCTP)
-15 - -30°C
Primer solution 300μL, Labeling Buffered ATP, dGTP and dTTP in Tris HCl pH7.5, 2-mercaptoethanol and MgCl2 600μL, Nucleotide solutions dATP, cCTP, dGTP, dTTP in Tris HCl pH 8, 0.5mM EDTA, Reaction Buffer A 10 x concentr
60 Reactions
Documents
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