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GE Healthcare BioTrak™ MMP-2 Activity Assay

Provides a simple, specific and precise quantitative determination of active or pro MMP-2 in tissue culture supernatants, serum, plasma, urine, synovial fluid and tissue homogenates

Manufacturer:  GE Healthcare RPN2631

Catalog No. 45-001-172


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Description

Description

  • Specific for Matrix Metalloproteinase-2 (MMP-2)
  • Either the endogenous (naturally occurring) or total (following activation using APMA) activity levels in samples can be detected
  • Range and sensitivity equivalent to ELISA
  • Non-radioactive coated microplate format for ease of use
  • Measures active MMPs from a range of species, including human, mouse, rat, rabbit and porcine
  • Applicable for complex biological samples such as serum, plasma, synovial fluids, tissue extracts, cell culture supernatants
  • Flexible protocol to cover speed and sensitivity requirements

Specifications

Specifications

MMP-2
Matrix Metalloproteinase-2, Biotrak Assay
66000
0.5 ng/ml (short incubation) 190 pg/ml (longer incubation)
-15 - -30°C
0.75 ng/ml/ (0.19 ng/ml possible with longer incubation)-12 ng/ml (3 ng/ml possibile with longer incubation)
ELISA
100 μl
The assay recognises the pro and active forms of MMP-2. It does not cross-react with other MMPs or TIMPs. Cross-reactivity has also been shown with rabbit, porcine, rat and mouse samples. Both pro and active MMP-2/TIMP-2 complexes as well as active MMP-2/
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