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GE Healthcare Biotrak™ MMP-9 Activity Assay

Quantitative measurement of endogenous active MMP-9 or total active MMP-9 (following activation using APMA)

Manufacturer:  GE Healthcare RPN2634

Catalog No. 45-001-248

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Description

Description

  • Range and sensitivity equivalent to ELISA
  • Range: 0.5-16ng/mL (1 hour incubation with detection reagent) 0.125-4 ng/mL (3.5 hour incubation with detection reagent)
  • Sensitivity: 0.125ng/mL
  • Overnight protocol

Specifications

Specifications

MMP-9
MMP-9, Biotrak Activity Assay
Microplate (12 x 8-well strip coated with Anti-MMP-9), 10mL Assay Buffer, 32ng Human pro MMP-9 standard (lyophilized), 352mg p-Aminophenylmercuric Acetate (APMA), 100μL detection Enzyme, Substrate (lyophilized), 12.5mL Wash Buffer
MMP-9 Biotrak activity assay
Tissue Culture Supernatants, Serum, Plasma, Synovial Fluid, Tissue Homogenates
The assay recognises the pro and active forms of MMP-9. It does not cross-react with other MMPs or TIMPs. Cross-reactivity has however been shown with rabbit and mouse samples. Both pro and active MMP-9/TIMP-1 complexes as well as active MMP-9/TIMP-2 comp
Overnight
0.5 ng/ml (0.125 ng/ml for longer incubations)-16 ng/ml (4 ng/ml for longer incubations)
Quantitative determination of active or pro MMP-9 in tissue culture supernatants, Serum, Plasma, Synovial fluid, tissue homogenates
86000
Each
0.5 ng/ml for the standard range
-15 - -30°C
Safety and Handling

Safety and Handling

ShelfLife : 1 Month

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