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GE Healthcare Butyl-S Sepharose™ 6 Fast Flow Chromatography Media

The least hydrophobic medium in the Sepharose Fast Flow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of purification process

Manufacturer:  GE Healthcare 17097802

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Catalog No. 45-002-941

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  • Binds and elutes relatively strong hydrophobic molecules at comparatively low salt concentrations
  • Hydrophilic nature of the base matrix ensures low levels of non-specific binding
  • Purifies recombinant Hepatitis B virus surface antigen from CHO cells
  • Composed of cross-linked 6% agarose beads modified with butyl groups via thio-ether linkage
  • Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development



250 to 400 cm/Hoµr
100 kPa
Stable in commonly used aqueous Buffers: 1 mM HCl, 1 M NaOH, 30% Isopropanol, 50% Ethylene Glycol, 70% Ethanol, 6 M Guanidine hydrochloride, 8 M Urea
4°C to 30°C
Butyl-S Sepharose 6 fast flow media
For Hydrophobic Medium in The Sepharose Fast Flow Hydrophobic Interaction Chromatography (HIC) Range and is Particularly Useful During The Initial Stages of PurifiCatIon Process
6% Cross-Linked Agarose
200 mL
20% Ethanol
45 - 165 µm
3 to 13
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