GE Healthcare Butyl-S Sepharose™ 6 Fast Flow Chromatography Media Available on GSA/VA Contract for Federal Government customers only.

The least hydrophobic medium in the Sepharose Fast Flow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of purification process

Manufacturer: GE Healthcare 17097802

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Catalog No. 45-002-941

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    Specifications

    Stability Stable in commonly used aqueous Buffers: 1mM HCl, 1M NaOH, 30% Isopropanol, 50% Ethylene Glycol, 70% Ethanol, 6M Guanidine hydrochloride, 8M Urea
    Quantity 200mL
    Storage Requirements 4 to 30°C
    Product Type Butyl-S Sepharose 6 fast flow media
    For Use With (Application) For Hydrophobic medium in the Sepharose Fast Flow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of purifiCatIon process
    Flow Rate 250 to 400cm/Hour
    Particle Size 45 to 165μm
    Max. Pressure 100kPa
    Matrix 6% Cross-Linked Agarose
    pH Range 3 to 13
    Storage Buffer 20% Ethanol
    Ligand Type Butyl-S

    • Binds and elutes relatively strong hydrophobic molecules at comparatively low salt concentrations
    • Hydrophilic nature of the base matrix ensures low levels of non-specific binding
    • Purifies recombinant Hepatitis B virus surface antigen from CHO cells
    • Composed of cross-linked 6% agarose beads modified with butyl groups via thio-ether linkage
    • Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development

  • Description & Specifications

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