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GE Healthcare Butyl Sepharose™ 4 Fast Flow Chromatography Media

Used for capture and intermediate purification of larger proteins

Manufacturer:  GE Healthcare 17098001

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Catalog No. 45-002-943


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Description

Description

  • Based on Sepharose Fast Flow base matrix derivatized via uncharged, chemically-stable ether linkages
  • Hydrophobic interaction chromatography (HIC) media composed of cross-linked 4% agarose
  • Aliphatic butyl ligand immobilized to base matrix with ether linkage
  • Fast Flow matrix gives the adsorbent high chemical, physical and thermal stabilities
  • Dynamic capacity at low salt concentration
  • Used with all aqueous buffers commonly used in chromatography
  • Autoclavable at 121°C for 20 minutes in water
  • Available in range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development

Specifications

Specifications

150 to 250 cm/Hoµr
9.84 in.
Butyl
100 kPa
Stable in commonly used aqueous Buffers: 1 M NaOH, 30% Isopropanol, 70% Ethanol, 6 M Guanidine-Hydrochloride, 30% Acetonitrile, 1 mM HCl
4°C to 30°C
Butyl Sepharose 4 Fast Flow Media
For Capture and Intermediate PurifiCatIon of Larger Proteins
25 cm
Based on cross-linked 4% agarose
200 mL
20% Ethanol
45 - 165 µm
3 to 13
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