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Cytiva Butyl Sepharose™ High Performance Chromatography Media

Designed for intermediate and polishing step purification steps

Manufacturer:  Cytiva 17543202

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Catalog No. 45-002-955

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  • Hydrophobic interaction chromatography (HIC) media composed of highly cross-linked 34μm agarose beads with aliphatic butyl functional groups
  • High-resolution, capacity separations for recovery
  • High ligand density for dynamic binding capacity even at low salt concentration
  • Display minimum of ionic interactions
  • Chemical stability for effective CIP and sanitization
  • Available in prepacked columns such as HiPrep™, HiScreen™, and HiTrap™ plus laboratory and BioProcess pack sizes



38 mg b-lactoglobulin/mL mediµm
For Intermediate and Polishing Step PurifiCatIon Steps
Cross-Linked Agarose
24 - 44 µm
200 mL
20% Ethanol
3 to 13
100 to 200 cm/Hoµr
300 kPa
High performance Butyl Sepharose media
Stable in commonly used aqueous Buffers: 1 M Sodium Hydroxide, 1 M Acetic Acid, 8 M Urea, 6 M Guanidine Hydrochloride, 30% Acetonitrile, 70% Ethanol, 3 M Ammonium sulfate, 1 mM HCl, 30% Isopropanol, 2% SDS
4°C to 30°C
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