Cytiva Capto™ Core 700

Capto Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules.

Supplier:  Cytiva 17548102

Catalog No. 45-002-596

Description

The novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality, size exclusion, and binding chromatography in one chromatography medium (resin).

• For intermediate purification and polishing of viruses and other large biomolecules (Mr > 700 000) in flowthrough mode.
• Novel core bead technology allows efficient capture of contaminants while target molecules are collected in the flowthrough.
• Significantly improved productivity and higher flow rates compared with gel filtration methods.
• Straightforward optimization due to flowthrough chromatography and robust performance.
• Convenient small-scale purification, process development, and scale-up using prepacked HiTrap and HiScreen columns.
• Capto Core 700 is composed of a ligand-activated core and inactive shell.
• The inactive shell excludes large molecules (cut off ∼ Mr 700000 [700 kDa]) from entering the core through the pores of the shell.
• These larger molecules are collected in the column flowthrough while smaller impurities bind to the internalized ligands.
• The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core.
• The multimodal ligands ensure strong binding with most impurities over a wide range of pH and salt concentrations.
• Bound impurities are removed from the beads by cleaning-in-place (CIP) procedures using NaOH and in most cases, a solvent.

Specifications

• Buffer: 1M NaOH, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol
• Quantity: 100mL
• Ligand Type: Octylamine
• Particle Size: 90μm
• Content And Storage: 4° to 30°C
• Matrix: Multimodal Resin

• Product Type: Capto Core 700 chromato graphy media
• Flow Rate: <500cm/hr.
• pH Range: 2 to 14
• Support Type: Highly Cross-linked Agarose
• For Use With (Application): Intermediate purification and polishing of viruses and other large biomolecules