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GE Healthcare Capto™ Core 700 Available on GSA/VA Contract for Federal Government customers only.

Capto Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules.

Manufacturer:  GE Healthcare 17548102

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Catalog No. 45-002-596

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The novel core bead technology and multimodal, octylamine ligand give Capto Core 700 dual functionality, size exclusion, and binding chromatography in one chromatography medium (resin).

  • For intermediate purification and polishing of viruses and other large biomolecules (Mr > 700 000) in flowthrough mode.
  • Novel core bead technology allows efficient capture of contaminants while target molecules are collected in the flowthrough.
  • Significantly improved productivity and higher flow rates compared with gel filtration methods.
  • Straightforward optimization due to flowthrough chromatography and robust performance.
  • Convenient small-scale purification, process development, and scale-up using prepacked HiTrap and HiScreen columns.
  • Capto Core 700 is composed of a ligand-activated core and inactive shell.
  • The inactive shell excludes large molecules (cut off ~ Mr 700000 [700 kDa]) from entering the core through the pores of the shell.
  • These larger molecules are collected in the column flowthrough while smaller impurities bind to the internalized ligands.
  • The core of each bead is functionalized with ligands that are both hydrophobic and positively charged, resulting in a highly efficient multimodal binding of various contaminants small enough to enter the core.
  • The multimodal ligands ensure strong binding with most impurities over a wide range of pH and salt concentrations.
  • Bound impurities are removed from the beads by cleaning-in-place (CIP) procedures using NaOH and in most cases, a solvent.

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1M NaOH, 6M Guanidine Hydrochloride, 30% Isopropanol and 70% Ethanol
2 to 14
Highly Cross-linked Agarose
Capto Core 700 chromato graphy media
Intermediate purification and polishing of viruses and other large biomolecules
4° to 30°C
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