GE Healthcare Cell Proliferation Kit, BioTrak™ ELISA Kit and Components Available on GSA/VA Contract for Federal Government customers only.

Provides a precise, fast and simple colorimetric assay to quantitate proliferating cells cultured in a 96 well microplate

Manufacturer: GE Healthcare RPN20

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Catalog No. 45-000-835

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This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000. 


    Specifications

    For Use With (Application) Immunocytochemistry
    Includes Labeling reagent 12mL, Nuclease (freeze-dried) 3 x 4mL when reconstituted, Anti-5-bromo-2'-deoxyuridine Monoclonal Antibody 120μL, Peroxidase Anti-Mouse IgG2a (concentrate) 180μL, Peroxidase Anti-Mouse IgG2a Diluent 12m
    Quantity 100 Slides
    Storage Requirements 2° to 8°C

    • Recommended cell number: 10e6-10e7 cells/mL (Cell proliferation kit) 10e2 cells/well-10e5 cells/well (Biotrak ELISA kit)
    • Required time: 2-3 hour post-labeling (labeling 1-2 hours)
    • Antibody and labeling reagent available separately for ELISA and ICC systems
    • Suitable for in vivo and in vitro tissue labeling in addition to cultured cells (ELISA format)
    • Non radioactive protocols provide sensitivity comparable to autoradiographic methods
    • Cell proliferation kit avoids the need to chemically denature DNA. Cellular morphology is preserved allowing multiple labeling experiments
    • 5-fluoro-2'-deoxyuridine is used to inhibit thymidilate synthetase increasing BrdU incorporation

    When ordering the Cell Proliferation Kit (RPN20), you must also order DAB REAGENT OF RPN20/RPN1174 (RPNK8888)

  • Description & Specifications

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