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GE Healthcare Con A Sepharose™ 4B Medium

An affinity chromatography media for capture of glycosylated biomolecules including glycoproteins and glycolipids

Manufacturer:  GE Healthcare 17044001

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Catalog No. 45-000-068


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Description

Description

Highly suited for isolation of cell surface glycoproteins from detergent-solubilized membranes.

  • Con A coupled to Sepharose, in a 4% agarose matrix via cyanogen bromide activation
  • Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues with available C-3, C-4, or C-5 hydroxyl groups
  • Group-specific adsorbent for molecules containing sugars
  • Concentration of immobilized Con A, approx. 13mg/mL medium; binding capacity for porcine thyroglobulin, approx. 30mg/mL medium
  • Stable in the pH range 4 to 9, and in the presence of all commonly used buffers
  • Average particle size: 90µm
  • Delivered in 0.1M acetate buffer, pH 6.0, containing 1M NaCl, 1mM CaCl2, 1mM MgCl2, 1mM MnCl2, and 20% ethanol

Specifications

Specifications

30mg/mL
Concanavalin A
4% Agarose
4 to 9
2° to 8°C
For separation and purification of Glycoproteins, Polysaccharides and Glycolipids, isolates cell surface Glycoproteins from detergent-solubilized membranes
13mg Con A/mL Drained Medium
90μm
100mL
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