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GE Healthcare GSTrap™ 4B Prepacked Columns: 16 x 25mm

For fast and simple purification of GST-tagged proteins

Manufacturer:  GE Healthcare 28401748

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Catalog No. 45-001-431

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  • Prepacked with Glutathione Sepharose™ 4B media, featuring a glutathione ligand coupled to 4% agarose
  • May be used with syringe, peristaltic pump or ¨A¨KTA design chromatography system
  • Purifies GST-tagged proteins from bacterial lysates and other glutathione S-transferases or glutathione-dependent proteins
  • Bed dimensions: 16 x 25mm
  • Flow velocity: <20mL/min.
  • Average particle size: 90µm
  • Matrix: Highly cross-linked 4% agarose
  • Binding capacity: >25mg horse liver GST/mL
  • pH stability working range: 4 to 13



5 mL
< 20 mL/min
5 x 5 mL
45 to 165μm
5 bar [0.5 MPa] (70 psi)
4 to 30°C, 20% Ethanol
0.62 in.
GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function
4 to 13
GSTrap 4B, 5 x 5 mL
5 × 5mL
4% Highly Cross-linked Agarose
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